WebDec 1, 2007 · Abstract. The mechanisms of the medium-mediated bystander response induced by gamma-rays in non-irradiated TK6 cells were investigated. Cell cultures were irradiated and the culture medium discarded immediately after irradiation and replaced with a fresh one. In cells incubated with conditioned medium from irradiated cells (CM), a … WebJan 1, 2016 · Abstract Closely related TK6, WTK1, and NH32 human B-lymphoblastoid cell lines differ in their p53 functional status. These lines are used frequently in genotoxicity studies and in studies aimed at understanding the role of p53 in DNA repair. Despite their routine use, little is known about the genetic status of these cells.
Development and validation of an in vitro micronucleus assay platform …
WebMar 11, 2024 · The human lymphoblastoid TK6 cell line was established in 1978 from the parent cell line WI-L2 that was derived from a 5-year-old male with hereditary spherocytosis (Lorge et al., 2016). Due to TK heterozygosity on human chromosome 17 in these cells as well as the presence of the hypoxanthine phosphoribosyltransferase ( HPRT ) gene on the … WebJan 17, 2024 · The TK6 cell line with wild type p53 function was used in our experiments. TK6 cells (human lymphoblastoid cells from spleen) were originally provided by the Tumorbank of the German Cancer Research Center, DKFZ, Heidelberg, Germany. DNA cell line authentication was done by Eurofins Medigenomix Forensik GmbH, Ebersberg, … explain what sql is used for
Microcolony Size Distribution Assay Enables High-Throughput Cell ...
WebNov 1, 2004 · Induced genomic instability in the human B lymphoblastoid cell line TK6 manifests itself as increases in end-to-end chromosome fusions and non-reciprocal chromosome translocations. It is not associated with elevated frequencies of specific locus mutations or other cytogenetic alterations. WebJul 4, 2012 · Abstract. The Organization for Economic Co-operation and Development (OECD) has recently adopted Test Guideline 487 (TG487) for conducting the in vitro micronucleus (MNvit) assay. The purpose of this study is to evaluate and validate treatment conditions for the use of p53 competent TK6 human lymphoblastoid cells in a TG487 … WebTK6 human lymphoblastoid cells are widely used in the in vitro MN assay as a part of regulatory genotoxicity testing for pharmaceuticals, but have seen relatively limited use in testing of other test materials, including tobacco-related products. As an established cell line, they do not exhibit the donor to donor variability observed in primary cells such as … bubba\u0027s philly cheese steak