Web2 µL x 10 micromolar (µM) ONT Strand-switching primer (SSP) 10 Mix gently by flicking the tube and spin down 00:00:05 11 Add the strand-switching buffer to the snap-cooled, annealed RNA, mix by flicking the tube and spin down 12 Incubate at 42 °C for 00:02:00 13 Add 1 µL of Maxima H Minus Reverse Transcriptase, to a total volume of 20 µL WebStrand switch models for generating QP mutations. The QP mutation illustrated is the most frequent event observed with the lys2ΔBgl allele (QP #1 in Figure 1A) and can be generated (A) via an ...
cDNA-PCR Sequencing Kit - Oxford Nanopore Technologies
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microRNA strand selection: Unwinding the rules - Medley - 2024
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