Pdx1 overexpression

Author: iwhp

August undefined, 2024

Splet27. jan. 2024 · Published: 27 January 2024 Controlled clustering enhances PDX1 and NKX6.1 expression in pancreatic endoderm cells derived from pluripotent stem cells … Splet15. feb. 2024 · Although PDX1 has already been reported as a potential cancer marker in CRC, it is based on the observation of PDX1 expression in cancer cells, and its role has …

Differentiation of immature enterocytes into enteroendocrine cells …

Splet05. feb. 2024 · Pancreatic and duodenal homeobox 1 (Pdx1) and Sonic hedgehog (Shh) are the key regulators of beta-cell function. In vitro experiments have shown that there is … Splet19. dec. 2013 · The ectopic overexpression of Pdx1, Neurog3, and MafA in mice reprograms acinar cells to insulin-producing cells. We developed a quantitative PCR … al115188

CircRNA circ-OGDH (hsa_circ_0003340) Acts as a ceRNA to …

Splet19. feb. 2024 · PDX1 overexpression plasmid (Flag- PDX1) was purchased from Origene Technologies (Origene). Alkaline phosphatase (ALP) activity. After treatment, each plate was fixed by 4% paraformaldehyde for 10 min and then washed with PBS for three times. Cells were stained with 5-bromo-4-chloro-3-indolylphosphate (BCIP) and 4-nitro blue … SpletHowever, overexpression of Pdx1 may facilitate the biosynthesis of insulin or the formation (or maturation) of insulin granules, because doxycycline treatment for 4 days also … Splet29. jan. 2024 · In addition, Pdx1 overexpression in vitro revealed that Pdx1 plays a role in maintaining β-cell phenotypes [20]. Thus, among the extensive in vitro and in vivo studies performed, there have been no direct in vivo studies showing whether the sustained expression of Pdx1 in β cells protects or improves β-cell function under diabetic … al1133

The Effect of Overexpression of Pdx1 and Foxa2 on the …

SpletWe transfected the pancreatic-duodenal homeobox 1 gene (Pdx1) into IEC-6 cells, an embryonic intestinal epithelial cell line derived from a normal rat, and selected the cells … Splet13. sep. 2011 · Overexpression of Pdx1 protein resulted in an upregulation of Ins1 and Ins2 mRNA expression seen as early as day 10 for Ins1 and day 14 for Ins2, and they reached … al116009Splet19. avg. 2014 · Pdx1诱导的肝细胞转分化研究进展分化,诱导,细胞,Pdx1,研究,肝细胞,研究进展,转分化,转分化,诱导分化 ... enase -2 ndits rela tionship eoloreeta onseiMed 95-5 Mtiller—Dec ker ，Neumam*et a1 ．Trans- genie eyelooxygenase-2 overexpression sensitizes *house skin carc inoge nesis． Proc Natl Muller-De cker a1 ... al1122 ifm

"Splet22. jun. 2012 · PDX1 overexpression was confirmed by Western blot ( Fig. 1 ). Anti-PDX1 antibody detects increased abundance of the roughly 45-kilodalton PDX1 protein in Caco-2 cells stably transfected with pmPDX1 compared to cells transfected with the empty vector. Download : Download full-size image Fig. 1. " - Pdx1 overexpression

Pdx1 overexpression

Tissue expression of PDX1 - Summary - The Human Protein Atlas

Splet27. nov. 2013 · Pancreatic and duodenal homeobox 1 (PDX1), a member of the homeodomain-containing transcription factor family, is a key transcription factor important for both pancreas development and mature β cell function. The ectopic overexpression of Pdx1, Neurog3, and MafA in mice reprograms acinar cells to insulin-producing cells. We … Splet06. jan. 2024 · Overexpression PDX1 could partially reverse the promotion effects of miR-1249-5p on osteogenesis of ADSCs. In conclusion, miR-1249-5p promotes osteogenic differentiation of ADSCs by targeting PDX1 through the PI3K/Akt signaling pathway. Osteoporosis (OP) is an age-related systemic bone disease. MicroRNAs (miRNAs) are …

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SpletThe serrated polyp-neoplasia pathway is a novel concept that has been demonstrated to differ from the conventional adenoma-carcinoma pathway. To characterize the phenotypic patterns of differentiation in colorectal serrated polyps, we examined the immunohistochemical expression profile of gastric (MUC5AC, TFF1, MUC6, GlcNAcα1 → … Splet04. apr. 2024 · PDX1 and ISL1 regulation of insulin gene expression in pancreatic beta cells, was investigated. Creating the Pdx1(DeltaAREAII) state after cells entered an insulin …

Splet01. jun. 2009 · Overexpression of Pdx1 in mouse pancreatic ductal cells (PDCs) was sufficient to activate endogenous Ngn3 mRNA levels as compared with cells transduced with the empty vector (Figure 5 B). Figure 5 Pdx1 regulates Ngn3, at least in part, via its direct occupancy of a conserved upstream enhancer of Ngn3. Splet13. jan. 2024 · The overexpression of Pdx1 plus Shh manipulation proved beneficial to the expression of critical genes involved in mature pancreatic β cells. Group M presented an important elevation in the expression of Pdx1 , MafA, Ngn3, Isl1, Nkx2.2 , and Nkx6.1 mRNA compared with group C. Group M showed a significantly higher expression of insulin …

Splet16. apr. 2024 · Second, we use ectopic gene overexpression and gene editing in cell culture to compare functional properties of PDX1 proteins. We show that the divergent gerbil … SpletPDX1 overexpression counteracted the inhibitory impact of miR-615-5p mimic on malignancy and glutamine metabolism of ESCC cells. Conclusion: Circ-OGDH sponged miR-615-5p to elevate PDX1 expression, thus elevating glutamine metabolism and promoting tumor growth in ESCC. The study offered evidence to support circ-OGDH as a promising …

Splet19. feb. 2014 · Overexpression of PDX1, NGN3, and MAFA has been successfully used to induce glucose responsiveness in hepatic stem cells and pancreatic acinar tissue [8, 9] and to alter the expression profile of thyroid NE cells. However, the effects induced by overexpression of PDX1 and MAFA were relatively small.

Splet21. dec. 2024 · PDX1 overexpression counteracted the inhibitory impact of miR-615-5p mimic on malignancy and glutamine metabolism of ESCC cells. Conclusion . Circ-OGDH sponged miR-615-5p to elevate PDX1 expression, thus elevating glutamine metabolism and promoting tumor growth in ESCC. The study offered evidence to support circ-OGDH as a … al112964Splet26. jul. 2024 · PDX1 overexpression was confirmed by RT-PCR and Western blotting. Effects of PDX1 ectopic expression on cell proliferation and motility were determined in PANC-1 cells using MTS, cell cycle analysis, transwell and wound-healing assay. EMT genes expression was analyzed in PDX1-overexpressing and Control PANC-1. al 1128Splet28. jan. 2024 · On the other hands, pancreatic and duodenal homeobox factor 1 (Pdx1) is an important transcription factor for various β-cell-related genes including insulin gene and thereby contributes to the maintenance of mature β-cell function. The aim of this study was to evaluate how Ffar1 expression in β-cells is altered under diabetic conditions. al 1123Splet02. jan. 2009 · Pdx1 is a major transcription factor involved in pancreatic development . It is expressed downstream of Foxa2, and its expression in the endoderm lineage is enriched … al116490Splet04. apr. 2024 · Pdx1 is a transcription factor required for the formation of endocrine pancreas that is inhibited during Coxsackievirus B4 infection of human pancreas ductal … al1163Splet19. jun. 2024 · 图2 腺病毒表达载体的包装及过表达效果检测Fig.2 Adenovirus expression vector packaging and overexpression effect detectionA、B：SOX9基因腺病毒表达载体在293A ... 其中，Pdx1基因在胚胎发育过程中能促进胰腺的早期发育和晚期胰岛素分泌细胞分化，在成体中能帮助维持胰岛β细胞的 ... al116980SpletPDX1 is an essential gene for pancreas formation, and PDX1 mutations in humans are associated with pancreatic agenesis/hypoplasia and neonatal diabetes (Piper et al., 2002; Stoffers et al., 1997; Thomas et al., 2009). From: Current Topics in Developmental Biology, 2024 View all Topics Add to Mendeley About this page al 116