Gfp mouse monoclonal antibody
WebThe blots were probed with mouse monoclonal anti-GFP antibodies for specific detection of the GFP-tagged coilin proteins (upper panel). The blots were re-probed with rabbit … WebThermo Fisher Scientific's GFP Antibody is a Mouse monoclonal antibody. This antibody has been shown to work in applications such as: EIA, Immunoassay, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry - fixed, and Western Blot.
Gfp mouse monoclonal antibody
Did you know?
WebApr 12, 2024 · GFP induction in C215 CAR-T cells was also seen after ... and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. J. Biol. WebMonoclonal antibody is produced by immunizing animals with native GFP. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
WebDec 6, 2024 · The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed. Recommended Assay Procedures WebRoche mouse monoclonal gfp antibody roche. Mouse Monoclonal Gfp Antibody Roche, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 …
WebGFP Dylight™ 488 Conjugated Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by ... WebThis antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with Rockland's polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or ...
WebDec 6, 2024 · Monoclonal antibody 1A12-6-18 reacts with Green Fluorescent Protein (GFP), which is a 27 kDa bioluminescent protein first purified from the jellyfish, Aequorea victoria …
WebLane B: GFP transfected 293 cell lysate (0.5mg) Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60μg of Immunomagnetic beads Protein G. Primary antibody: Anti-GFP Tag rabbit monoclonal antibody, at 1/1000-1/5000 dilution. Secondary. Goat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution. Developed using … examples of a boneWebOur GFP polyclonal, monoclonal and recombinant monoclonal antibodies are developed in Mouse, Rabbit, Goat, Chicken, Rat and Alpaca. These antibodies have been verified … brushed nickel and gold bathroom faucetshttp://biopioneer.com.tw/?p=41026 examples of absorption in scienceWebPurified His-tagged GFP protein was used to produced this monoclonal antibody. Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is … examples of absolute monarchy governmentWebAnti-GFP is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent preparation. Purity: Both Anti-GFP mouse monoclonal antibodies (Clones 7.1 and 13.1) are >95% pure as determined by SDS-PAGE and ion-exchange HPLC analyses. Preparation Note brushed nickel and gold cabinet pullsWebDec 6, 2024 · Monoclonal antibody 1A12-6-18 reacts with Green Fluorescent Protein (GFP), which is a 27 kDa bioluminescent protein first purified from the jellyfish, Aequorea victoria … examples of absolutism in ethicsWebGFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally … brushed nickel angle iron