WebThe present invention relates to a method of Agrobaterium-based transformation of a plant comprising: generating a callus explant by, performing callus induction and maintenance in a plant in at least a first solution comprising less than about 100 mg/l of inositol; subjecting the callus explant to a cold treatment in a second solution comprising glutamine; and co … WebDec 1, 2024 · Four of the most frequently used strains, GV3101, EHA101, EHA105, and AGL-1, are C58 derivatives and, as such, C58 is by far the most commonly used …
Strain specific Agrobacterium -mediated genetic transformation of ...
WebJun 28, 2016 · An effective transformation protocol for Dunaliella, a β-carotene producer, was developed using the synergistic mechanism of D-glucose and Acetosyringone on three different Agrobacterium strains (EHA105, GV3101 and LBA4404). In the present study, we investigated the pre-induction of Agrobacterium strains harboring pMDC45 binary … WebOct 16, 2024 · The Agrobacterium strain LBA4404, GV3101 and EHA105 competent cells (Weidi Bio Tech, Shanghai, China) stored at − 80 were thawed on ice and added 5 μL recombined plasmids, then kept in ice for 5 min. Then the mixture was fast frozen in liquid nitrogen for 5 min, followed by an incubation at 37 ℃ for 5 min. ottawa marriott tripadvisor
An improved and efficient method of Agrobacterium syringe …
WebMar 24, 2024 · Gene, strain, binary vector, and Agrobacterium culture. For transformation studies, Agrobacterium tumefaciens strain EHA105 harboring the binary vector pCAMBIA2301 was used. The T-DNA region carries uidA or GUS–A reporter gene and was initiated by CaMV35S promoter and NOS terminator sequences, and the kanamycin … WebAgrobacterium Competent Cells. Using Propriety methods, Intact Genomics has developed and manufactured a variety of Agrobacterium chemically and electroporation competent cells with high efficiency. Agrobacterium strains include EHA105, LBA4404, GV3101 and AGL-1. All are useful for plant transgenic operations in a variety of species. WebMar 6, 2024 · Two Agrobacterium strains, EHA105 and LBA4404, both harboring the p35SGUSINT plasmid, carrying the reporter gene for β-glucuronidase ( gus) and the marker gene neomycin phosphotransferase ( nptII ), were used to test the efficiency of the process. イオンクレジットサービス株式会社 userid aeon.co.jp