WebAug 21, 2024 · The assembly of multi-fragments was achieved by ClonExpress™ II One Step Cloning Kit or ClonExpress MultiS One Step Cloning Kit (Vazyme Biotech Co., Ltd., China). Plasmid DNA and yeast genomic DNA were obtained using TIANprep Rapid Mini Plasmid Kit (TIANGEN) and TIANamp Yeast DNA Kit (TIANGEN), respectively. Strains … WebClonExpress MultiS One Step Cloning Kit. 非连接酶依赖型的多片段一步克隆技术. 快速克隆；高通量克隆；无缝克隆；DNA定点突变；双链DNA与载体组装. · 可在几乎任何载体的任意位点进行多片段拼接克隆. · 无需考虑 …

C113-Clon Express MultiS One Step Cloning Kit - IA Rugby.com

Web载体序列信息及测序方案. pCE-Zero vector载体，测序时使用通用测序引物（M13）即可。. ClonExpress ® Ultra One Step Cloning Kit是新一代的重组克隆试剂盒，可快速高效的将1-5个片段定向克隆至任意载体的任意位点，高度优化的2×ClonExpress Mix，50℃反应5-15 min完成重组，操作 ... WebEfficient cloning of 50 bp-10 kb DNA fragments; Linearized cloning vector and PCR products can be cloned directly without purification; No ligase, no self-connection, … ea-300 maiden flight

ClonExpress MultiS 一步克隆试剂盒 C113 - Vazyme Biotech

WebVazyme Biotech Co cloneexpress ii multis one step cloning kit. Cloneexpress Ii Multis One Step Cloning Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz … WebClonExpress II One Step Cloning Kit (Vazyme, Nanjing, China), both gene fragments were ligated to produce the plasmid pP43NMK-EAK16-E3C/E55F/D213T expressing E3C/E55F ... WebFeb 9, 2024 · following the instruction of the ClonExpress II One Step Cloning Kit (Vazyme, China), generating mini-CTX-Ptat-lacZ. The I-F cas operon including its native promoter (8.693-kb) was obtained by PCR employing PA154197 genomic DNA as the template. The DNA fragment was inserted into the KpnI site in the mini-CTX-Ptat-lacZ … ea3600f40b