Checkerboard dna–dna hybridization
WebFeb 19, 2007 · DNA–DNA hybridization showed that the amplified genome of each species used either as target or as probe provided signals equivalent to controls and that amplification of a mixture of species provided signals comparable to those provided by … WebHybridiza-tion signals are detected using chemifluorescence procedures. Bacterial numbers are determined by analyzing hybridization signals using a Storm system. A genus-specific probe is used to assess the total number of streptococci and universal …
Checkerboard dna–dna hybridization
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WebJul 15, 2008 · The checkerboard DNA–DNA hybridization technique enables the simultaneous identification of distinct bacterial species in a large number of dental samples. The original technique employed digoxigenin-labeled whole genomic DNA probes which … WebFeb 25, 2024 · Aims To evaluate the microbiota of endodontic infections in deciduous teeth by checkerboard DNA-DNA hybridization after uniform amplification of DNA in samples by multiple displacement amplification (MDA). Methodology Forty samples from the root …
WebThe presence and levels of 47 bacterial species were determined by checkerboard DNA-DNA hybridization. All 15 patients (10 had severe immunodeficiency) had been infected sexually. Thirteen patients were taking antiretroviral medication (66.7% undergoing highly active antiretroviral therapy). Regarding necrotizing periodontal diseases ... WebThe Checkerboard DNA-DNA hybridization is a technique that provides a simultaneous quantitative analysis of microbial species against mixed microbiota samples on a single membrane using whole-genome DNA probes. This molecular method was initially developed to study dental plaque microorganisms involved in periodontitis.
WebCheckerboard DNA-DNA Hybridization Technology Using Digoxigenin Detection. Lisa S. Gellen, Glenn M. Wall-Manning, Chris H. Sissons; Pages 39-67. ... Combined with the excellent overviews on microarrays as well as hybridization and detection techniques, and several useful assays for the application of these techniques for nucleic acid analysis ... WebJun 1, 2000 · The results of the checkerboard DNA-DNA hybridization analysis showed that 22 of the 42 DNA probes tested were reactive with 1 or more samples. The number of bacterial species in the root canal samples ranged from 1 to 17 (mean, 4.7). Seventeen of the 28 root canal samples were positive for at least 1 DNA probe.
WebStarting in 1994 with the DNA-DNA checkerboard technique developed by Sigmund Socransky that enabled the simultaneous identification of 43 …
WebJun 11, 2024 · DNA-DNA hybridization A technique that is used to compare DNA from two different species, to locate or identify nucleotide sequences, and to establish the effective in vitro transfer of nuclear material to a new host. how to determine what size furnace is neededWebIn genomics, DNA–DNA hybridization is a molecular biology technique that measures the degree of genetic similarity between pools of DNA sequences. It is usually used to determine the genetic distance between two organisms and has been used extensively … how to determine what size scope rings to buyWebDec 1, 2004 · Digoxigenin-labeled whole genomic probes to 40 common subgingival species were tested in a checkerboard hydridization format. Chemifluorescent signals resulting from the hybridization reactions were quantified using a Fluorimager and used to evaluate … how to determine what size dehumidifierWebFeb 5, 2008 · The checkerboard DNA–DNA hybridization technique was previously described (Socransky et al., 1994). In brief, the samples were lysed and the DNA placed in lanes on a nylon membrane using a Minislot device. After fixation of the DNA to the membrane, the membrane was placed in a Miniblotter 45 with the lanes of DNA at 90° to … how to determine what size generator neededWebCheckerboard DNA-DNA hybridization (CKB) is a technique that provides a simultaneous quantitative analysis of 40 microbial species against up to 28 mixed microbiota samples on a single membrane; using digoxigenin (DIG)-labeled, whole-genome DNA probes. how to determine what size prop for my boatWebJun 1, 2000 · The checkerboard DNA-DNA hybridization method has been reported to have a detection limit of 103 to 104 cells, depending on the species in ques- tion.12 It is possible that some microorganisms were present at levels <104, which was below the … the movie croods 2WebMar 30, 2024 · Methods: Unstimulated saliva samples were collected from 229 OSCC-free and 45 OSCC subjects and evaluated for their content of 40 common oral bacteria using checkerboard DNA-DNA hybridization. DNA counts per ml saliva were determined for each species, averaged across subjects in the 2 subject groups, and significance of … the movie cry macho